Publications

Highlights

Injectable osteogenic microtissues containing MSCs conformally fill and repair critical-size defects

We present a biomaterials-based strategy combining mesenchymal stromal cells (MSC) with a chitosan-collagen matrix to form modular microtissues designed for delivery through a needle to conformally fill cavital defects. Implantation of microtissues into a calvarial defect in the mouse resulted in complete bridging of the cavity.

Annamalai RT, Hong, Schott, Tiruchinapally, Levi and Stegemann

Biomaterials, 2019; 208:32-44

Harnessing macrophage mediated pathways for degradation of gelation microspheres for Spatiotemporal Control of BMP2 Release

We developed genipin-crosslinked gelatin microspheres designed to be preferentially degraded by inflammatory (M1) macrophages to provide better spatiotemporal control of BMP2 delivery for recalcitrant wounds.

Annamalai RT, Turner, Carson, Kunkel, Levi and Stegemann

Biomaterials, 2018; 161:216-227

Bioresponsive Microspheres for On-demand Delivery of Anti-inflammatory Cytokines for Articular Cartilage Repair

Here we describe the utility of gelatin microspheres that are responsive to arthritic flares, resulting in on-demand, and spatiotemporally controlled release of anti-inflammatory cytokines for cartilage preservation and repair.

Park, Hart, Rolauffs, Stegemann, and Annamalai RT

bioRxiv, 2019; 636886

Vascular network formation by microvascular endothelial cells in modular fibrin microtissues

We investigated a modular tissue engineering approach to therapeutic revascularization using fibrin-based microtissues containing embedded human endothelial cells and human fibroblasts. Robust sprouting from microtissues was evident, with larger vessels developing over time and FB acting as pericyte-like cells by enveloping endothelial tubes. This modular format may allow minimally invasive delivery of populations of prevascularized microtissues for therapeutic applications.

Annamalai RT, Rioja, Putnam and Stegemann

ACS Biomaterials, 2016; 2(11):1914-25

Transport Analysis of Engineered Liver Tissue Fabricated Using a Capsule-Based, Modular Approach

Previously, we demonstrated assembly of a prototype, engineered liver tissue, formed by the fusion of hepatocyte-containing capsules. Here, we analyzed the effects of various controllable system parameters with the aim of predicting the operating limits of our modular tissue in high cell density, perfused cultures.

Annamalai RT and Matthew HW

Ann Biomed Eng, 2019; 47(5):1223-36

Biofabrication of injectable fibrin microtissues for minimally-invasive therapies-Application of surfactants

Microtissues created from the protein fibrin and containing embedded cells can be used in modular tissue engineering approaches to create larger, hierarchical and complex tissue structures. In this paper we demonstrate an emulsification-based and the beneficial effects of surfactants in reducing microtissue aggregation.

Annamalai RT, Naik, Prout, Putnam, and Stegemann

Biomedical Materials, 2018; 13:045005

Collagen Type II enhances chondrogenic differentiation in agarose-based nodular microtissues

We developed modular microenvironments that mimic the composition of articular cartilage as a delivery system for consistently differentiated cells. We showed that the Inclusion of collagen Type II in agarose matrices in microbead format can potentiate chondrogenic differentiation of human MSC.

Annamalai RT, Mertz, Daley and Stegemann

Cytotherapy, 2016; 18(2):263-77

A glycosaminoglycan based, modular tissue scaffold system for rapid assembly of perfusable, high cell density, engineered tissues

In this paper, we report the elements of a simple yet efficient method for fabricating vascularized tissue constructs by fusing biodegradable microcapsules with tunable interior environments. A prototype, engineered liver tissue, formed by fusion of hepatocyte-containing capsules exhibited urea synthesis rates and albumin synthesis rates comparable to standard collagen sandwich hepatocyte cultures.

Annamalai RT, Armant and Matthew

PLoS One, 2014; 9(1):e84287

 

Full List

ORCID iD iconhttps://orcid.org/0000-0002-9880-3973

Injectable osteogenic microtissues containing MSCs conformally fill and repair critical-size defects
Annamalai RT, Hong, Schott, Tiruchinapally, Levi and Stegemann
Biomaterials, 2019; 208:32-44

Harnessing macrophage mediated pathways for degradation of gelation microspheres for Spatiotemporal Control of BMP2 Release
Annamalai RT, Turner, Carson, Kunkel, Levi and Stegemann
Biomaterials, 2018; 161:216-227

Bioresponsive Microspheres for On-demand Delivery of Anti-inflammatory Cytokines for Articular Cartilage Repair
Park, Hart, Rolauffs, Stegemann, and Annamalai RT
bioRxiv, 2019; 636886

Vascular network formation by microvascular endothelial cells in modular fibrin microtissues
Annamalai RT, Rioja, Putnam and Stegemann
ACS Biomaterials, 2016; 2(11):1914-25

Transport Analysis of Engineered Liver Tissue Fabricated Using a Capsule-Based, Modular Approach
Annamalai RT and Matthew HW
Ann Biomed Eng, 2019; 47(5):1223-36

Biofabrication of injectable fibrin microtissues for minimally-invasive therapies-Application of surfactants
Annamalai RT, Naik, Prout, Putnam, and Stegemann
Biomedical Materials, 2018; 13:045005

Multimode ultrasound viscoelastography for interrogation of mechanical properties in heterogeneous biomaterials
Hong X, Annamalai RT, Kemmerer T, Deng C, Stegemann
Biomaterials, 2018; 178:11-22

Collagen Type II enhances chondrogenic differentiation in agarose-based nodular microtissues
Annamalai RT, Mertz, Daley and Stegemann
Cytotherapy, 2016; 18(2):263-77

A glycosaminoglycan based, modular tissue scaffold system for rapid assembly of perfusable, high cell density, engineered tissues
Annamalai RT, Armant and Matthew
PLoS One, 2014; 9(1):e84287

Endothelial sprouting and network formation in collagen- and fibrin-based modular microbeads
Rioja AY, Annamalai RT, Spencer, Putnam and Stegemann
Acta Biomaterialia, 2016; 29:33-41

Evaluation of salivary cytokines for diagnosis of both trauma-induced and genetic heterotopic ossification
Sung, Chung, Habbouche, Cholok, Allen, Annamalai RT, Priest, Loder, Li, Stegemann, Kunkel and Levi
Front Endocrinol, 2017; 8:74